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This Ti2 system has 3-D STORM capability, which will enable users to image with 20nm lateral and 50nm axial resolution. This can also be used for standard total internal reflection fluorescence (TIRF) imaging on an Andor 897 EMCCD camera. The system also has a Yokogawa-X1 spinning disk confocal system with a Photometrics Prime 95B back-thinned CMOS camera. Finally, to enable optogenetic manipulations, this system also has a Mightex Polygon DMD that allows for the precise activation or uncaging of light-sensitive proteins or molecules. For maintaining live cells, this system has an Tokai HIt stage-top incubation system and a Mad City Labs piezo Z stage for fast and precise Z-stack acquisition.

WHAT IS IT GOOD FOR?

This system is excellent for anyone doing live cell imaging. The spinning disk cofocal breaks up a single laser line into hundreds of points, making it much more gentle than point-scanning confocals. Since it has pinholes it maintains the excellent Z-resolution of a scanning confocal, and with a fast Z-stage it can acquire 10 µm Z-stacks in less than a second. Additionally, the total internal reflection fluorescence (TIRF) modality is useful for live cell imaging of processes that occur near to the basal side of the cell while also being very gentle because it uses an evanescent wave to illuminate the specimen rather than direct laser excitation. Since the evanescent wave does not penetrate far into cells and only excites fluorescent molecules that are close to the bottom of the cells, TIRF microscopy has a very high signal to noise ratio. The STORM imaging mode can also be used with fixed specimens and provides exceptional resolution, down to 20nm. For more information about these imaging modes, please see:

http://zeiss-campus.magnet.fsu.edu/articles/spinningdisk/introduction.html

https://www.microscopyu.com/techniques/fluorescence/total-internal-reflection-fluorescence-tirf-microscopy

https://www.microscopyu.com/techniques/super-resolution/single-molecule-super-resolution-imaging

STERLING - OUR SWISS ARMY KNIFE SCOPE

STERLING - OUR SWISS ARMY KNIFE SCOPE