Notes on Imaging Chambers and glass

When you are preparing samples to bring to the NIC, please ensure that your cover glasses and or glass-bottom chambers are #1.5 glass. This glass is 170µm thick (high quality glass will have a tolerance of ±5µm) and most microscope objectives are designed for this glass thickness. In fact the coverslip is usually the first lens considered when the lightpath is designed. The thickness of the coverglass is extremely important for using high NA oil-immersion objectives. Multiple companies supply high-tolerance #1.5 coverslips and imaging chambers. #0 and #1 coverslips should only be used when extra working distance is absolutely essential, and when they are used, a member of the staff should be notified so that an objective can be placed onto the system to compensate for the non-standard coverslip thickness.

Additionally, live cell imaging chambers that are compatible with our incubation systems are 35mm dishes, and Lab-Tek II Chambered Coverglasses. ibidi chambers, with a footprint similar to a standard micrsocopy side are also compatible with our stage-top incubators.


Immunofluorescence Pointers

Immunofluorescence is a


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