This Ti2 system has a point-scanning confocal that has combination scanhead that can image with either galvo mirrors or high-speed resonant scanning mirrors and can use either a four-channel (two PMT and two GaAsP) detector or a spectral detector. The scanner unit can also use it’s resonant-scanning mirrors with it’s galvo-scanning mirrors to perform simultaneous photobleaching and imaging. This system also has a structured-illumination imaging (N-SIM) system that can resolve down to 115nm, twice the resolving power of a standard microscope, in TIRF, 2-D and 3-D modes. This system has an Okolab stage-top incubation system.

WHAT IS IT GOOD FOR?

The A1R is a resonant scanning confocal, which means that it can image with very high speed for a point-scanning confocal (easily 4 frames per second). So, it is useful for imaging live cells as well as fixed specimens. Also, since it has two pairs of scanning mirrors, we can use one for imaging while we use the second for photobleaching or uncaging, which means that we can visualize all of the kinetics of recovery after a region is photobleached. This system has a spectral detector that allows users to visualize the emission spectrum of their fluorophores. This allows us to image fluors which are very close spectrally and then to unmix the resulting emission. Finally, the structured illumination mode can acquire images of fixed and live cells with two times the resolution of traditional microscopy modes.

FOR MORE INFORMATION SEE -

https://www.microscopyu.com/techniques/confocal/resonant-scanning-in-laser-confocal-microscopy

https://www.microscopyu.com/techniques/confocal/spectral-imaging-and-linear-unmixing

https://www.microscopyu.com/tutorials/resonant-scanning-confocal-microscope-zoom

https://www.ibiology.org/talks/structured-illumination-microscopy/

Lana better.jpg